Are Aggregates of Enzyme Molecules More Effective than Individual Enzyme Molecules?
نویسندگان
چکیده
منابع مشابه
Allosteric inhibition of individual enzyme molecules trapped in lipid vesicles.
Enzymatic inhibition by product molecules is an important and widespread phenomenon. We describe an approach to study product inhibition at the single-molecule level. Individual HRP molecules are trapped within surface-tethered lipid vesicles, and their reaction with a fluorogenic substrate is probed. While the substrate readily penetrates into the vesicles, the charged product (resorufin) gets...
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ژورنال
عنوان ژورنال: Molecular Enzymology and Drug Targets
سال: 2016
ISSN: 2572-5475
DOI: 10.21767/2572-5475.10018